hilymax (Dojindo Labs)
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hilymax
Hilymax, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 668 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hilymax/HilyMax/pm42118211-77-8-9
Average 96 stars, based on 668 article reviews
Hilymax, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 668 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hilymax/HilyMax/pm42118211-77-8-9
Average 96 stars, based on 668 article reviews
hilymax - by Bioz Stars,
2026-09
96/100 stars
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Transfection:Article Title: A conformation-dependent hydrophobic degron determines Rab9a-mediated vesicular trafficking. Article Snippet: Mycoplasma-free HeLa cells were purchased from RIKEN BRC cell bank and American Type Culture Collection (ATCC) and were cultured in Dulbecco’s modified Eagle’s medium (Sigma Chemical Co., St. Louis, MO) supplemented with 5% heat-inactivated fetal bovine serum (FBS) at 37°C in a 5% CO2 atmosphere. .. Transfection of HeLa cells with pCIneo expression vectors was performed by using PEI MAX (Polysciences, Inc, Warrington, PA) or Article Title: A conformation-dependent hydrophobic degron determines Rab9a-mediated vesicular trafficking Article Snippet: Mycoplasma-free HeLa cells were purchased from the RIKEN BRC Cell Bank and the American Type Culture Collection and were cultured in Dulbecco’s modified Eagle’s medium (Sigma Chemical Co) supplemented with 5% heat-inactivated fetal bovine serum at 37 °C in a 5% CO 2 atmosphere. .. Transfection of HeLa cells with pCI-neo expression vectors was performed by using PEI MAX (Polysciences, Inc) or Article Title: Development of venus-fused Cypridina luciferase (VCL) and specific nanobody-tethering assay to model kleptoprotein uptake. Article Snippet: Human embryo kidney cells (HEK293T) (ECACC, 12,022,001, KAC, Hyogo, Japan) were cultured in high-glucose DMEM (Fujifilm Wako) supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific) and 1% penicillin–streptomycin (Fujifilm Wako) at 37 °C in a humidified atmosphere with 5% CO2. .. Cells were transfected with pEF-Nanobody-FLAG-2A-mCherry (50–1000 ng/mL) using Article Title: Antibodies for detection of Article Snippet: .. Other methods of transfection include proprietary transfection reagents such as LIPOFECTAMINE (Thermo Fisher Scientific, Inc., Waltham, MA), Article Title: Effects of Cyanidin Derivatives for the Aggregation of Cu/Zn Superoxide Dismutase 1 Article Snippet: .. After 24 h, the plasmid was transfected using Article Title: Induction of Hepatocyte Growth Factor Production by Quercetin. Article Snippet: Horseradish peroxidase (HRP)-conjugated anti-rabbit (Cell Signaling Technology) or anti-mouse secondary antibodies (Cell Signaling Technology) were used at a 1 : 3000 dilution. .. Immunoreactive bands were visualized using immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using LuminoGraphI (WSE-6100, ATTO Corporation, Tokyo, Japan). cAMP Response Element (CRE) Reporter Assay pcDNA3.1-β2AR was constructed by SLiCE as mentioned in a previous study.14) HEK293T cells were transfected with pGL4.29 (Promega Corporation, Madison, WI, U.S.A.) and pcDNA3.1-β2AR using Expressing:Article Title: A conformation-dependent hydrophobic degron determines Rab9a-mediated vesicular trafficking. Article Snippet: Mycoplasma-free HeLa cells were purchased from RIKEN BRC cell bank and American Type Culture Collection (ATCC) and were cultured in Dulbecco’s modified Eagle’s medium (Sigma Chemical Co., St. Louis, MO) supplemented with 5% heat-inactivated fetal bovine serum (FBS) at 37°C in a 5% CO2 atmosphere. .. Transfection of HeLa cells with pCIneo expression vectors was performed by using PEI MAX (Polysciences, Inc, Warrington, PA) or Article Title: A conformation-dependent hydrophobic degron determines Rab9a-mediated vesicular trafficking Article Snippet: Mycoplasma-free HeLa cells were purchased from the RIKEN BRC Cell Bank and the American Type Culture Collection and were cultured in Dulbecco’s modified Eagle’s medium (Sigma Chemical Co) supplemented with 5% heat-inactivated fetal bovine serum at 37 °C in a 5% CO 2 atmosphere. .. Transfection of HeLa cells with pCI-neo expression vectors was performed by using PEI MAX (Polysciences, Inc) or other:Article Title: High-throughput assessment of vocal modulation following chemogenetic inhibition in songbirds Article Snippet: Plasmid Preparation:Article Title: Effects of Cyanidin Derivatives for the Aggregation of Cu/Zn Superoxide Dismutase 1 Article Snippet: .. After 24 h, the plasmid was transfected using Western Blot:Article Title: Induction of Hepatocyte Growth Factor Production by Quercetin. Article Snippet: Horseradish peroxidase (HRP)-conjugated anti-rabbit (Cell Signaling Technology) or anti-mouse secondary antibodies (Cell Signaling Technology) were used at a 1 : 3000 dilution. .. Immunoreactive bands were visualized using immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using LuminoGraphI (WSE-6100, ATTO Corporation, Tokyo, Japan). cAMP Response Element (CRE) Reporter Assay pcDNA3.1-β2AR was constructed by SLiCE as mentioned in a previous study.14) HEK293T cells were transfected with pGL4.29 (Promega Corporation, Madison, WI, U.S.A.) and pcDNA3.1-β2AR using Reporter Assay:Article Title: Induction of Hepatocyte Growth Factor Production by Quercetin. Article Snippet: Horseradish peroxidase (HRP)-conjugated anti-rabbit (Cell Signaling Technology) or anti-mouse secondary antibodies (Cell Signaling Technology) were used at a 1 : 3000 dilution. .. Immunoreactive bands were visualized using immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using LuminoGraphI (WSE-6100, ATTO Corporation, Tokyo, Japan). cAMP Response Element (CRE) Reporter Assay pcDNA3.1-β2AR was constructed by SLiCE as mentioned in a previous study.14) HEK293T cells were transfected with pGL4.29 (Promega Corporation, Madison, WI, U.S.A.) and pcDNA3.1-β2AR using Construct:Article Title: Induction of Hepatocyte Growth Factor Production by Quercetin. Article Snippet: Horseradish peroxidase (HRP)-conjugated anti-rabbit (Cell Signaling Technology) or anti-mouse secondary antibodies (Cell Signaling Technology) were used at a 1 : 3000 dilution. .. Immunoreactive bands were visualized using immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using LuminoGraphI (WSE-6100, ATTO Corporation, Tokyo, Japan). cAMP Response Element (CRE) Reporter Assay pcDNA3.1-β2AR was constructed by SLiCE as mentioned in a previous study.14) HEK293T cells were transfected with pGL4.29 (Promega Corporation, Madison, WI, U.S.A.) and pcDNA3.1-β2AR using |